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TSH stimulates cAMP-dependent MEK and ERK activation. Starved WRT cells were stimulated with TSH (A) or forskolin (fsk) (B) for the times indicated (in minutes). C, cells were pretreated with U0126 (10 μM) for 30 min before stimulation. Total cell lysates were analyzed for ERK and MEK activation by Western blotting for phospho-ERK (ERK-P) and phospho-MEK1/2 (MEK-P). Equal protein loading was confirmed by Western blotting for Rac1 or ERK2.
