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ERK activation in FRTL5 and PCCL3 cells does not require Rap activity. FRTL-5 (A) and PCCL3 cells (B) infected with Rap1GAP adenovirus (500 particles/cell) were starved for 24 h, stimulated with forskolin for the indicated times (in minutes), and subjected to Western blotting for ERK phosphorylation, Rap1GAP, and Akt expression, the latter to confirm equal protein loading. Similar results were obtained in two experiments in each cell line.
