Fig. 7.
Caveolin-1 knockout increases fluoride and forskolin-stimulated adenylyl cyclase activity in mouse striatum. Ten- to 12-week-old wild-type or caveolin-1 knockout littermate mice were colony bred, brains were obtained, and striatum was microdissected. 10 μg of isolated striatum membranes were assayed for adenylyl cyclase activity. A, striatum membranes from wild-type or caveolin-1 knockout mice were incubated for 20 min, with a reaction mixture including [32P]ATP and ±10 mM sodium fluoride/20 μM aluminum chloride. [32P]cAMP was isolated and determined by scintillation counting, normalized to time and milligrams of membrane protein. B, striatum membranes were incubated with ± 100 μM forskolin for 20 min [32P]cAMP was isolated and determined by scintillation counting, normalized to time and milligrams of membrane protein. Data are the means ± S.E.M. from four littermate mice pairs (n = 4) assayed in triplicate. *, p < 0.05 versus wild-type mice for each treatment group.