Fig. 1.

A, confocal images of plasma membrane nucleolin in MV4-11 cells. Nucleolin localization was determined by indirect immunofluorescence using an anti-nucleolin primary antibody and an FITC-conjugated secondary antibody. Colocalization of nucleolin staining with the cell membrane marker dye, FM 4-64, demonstrates the presence of nucleolin in the plasma membrane of MV4-11 cells. The magnification used to capture the images was 88×. Results from two separate experiments are shown. B, effect of AS1411 and CRO-26 on the growth (cell counting) and viability [ 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay] of MV4-11 and K-562 cells. ♦, untreated control; ○, 10 μM CRO-26; ▴, 5 μM AS1411; ●, 10 μM AS1411; ■, 20 μM AS1411.