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. 2009 Nov;23(11):3712–3721. doi: 10.1096/fj.09-131607

Figure 1.

Figure 1.

ALK-1 specifically interacts with CK2β. A) COS-7 cells were transfected with FLAG-CK2β and HA-ALK-1. After 48 h, cells were lysed in lysis buffer, immunoprecipitated with HA or FLAG antibodies as indicated, resolved by SDS-PAGE, and immunoblotted with HA or FLAG antibodies as indicated. B, C) HMEC-1 cells were nucleofected with HA-ALK-1. After 48 h, cells were lysed, immunoprecipitated with either HA antibody or CK2β-specific antibody, and the immunoprecipitates analyzed on SDS-PAGE followed by immunoblot analysis with HA or CK2β antibody. Portions of the lysates were analyzed by immunoblot for the indicated proteins for expression, and β-actin was assessed as loading control. D) HMEC-1 cells expressing HA-ALK-1 were fixed and probed with HA antibody (left panel) and CK2β-specific antibody (middle panel). Arrows along the cell periphery indicate their colocalization (merged image, right panel). Data are representative of 3 independent experiments.