Figure 2.

Effects of C5aR-siRNA silencing on intensity of IgGIC-induced lung injury. A) Four hours after the onset of IgGIC-induced injury, BAL fluids were harvested, and mouse albumin levels in the BAL fluids were measured by ELISA as an index of vascular leakage; n = 5 mice/group. B) Trace amounts of ¹²⁵I-labeled BSA were added to the unlabeled BSA and injected intravenously to induce the IgGIC-induced lung injury. Albumin leakage into the lung (permeability index) was determined 4 h after the injury and expressed as the ratio of ¹²⁵I-BSA in perfused lung to that in 100 μl serum obtained at the time of sacrifice; n = 5 mice/group.