Figure 3.

Effects of C5aR-siRNA silencing on lung MPO content and BAL levels of TNF-α, IL-6, and KC. Mice received adenovirus 4 d before induction of acute lung injury. Whole-lung homogenates were analyzed for MPO activity 4 h after the onset of injury. A–D) BAL fluids obtained 4 h after the onset of injury were analyzed for expression levels of MPO (A), TNF-α (B), IL-6 (C), and KC (D) by ELISA. E) Alveolar macrophages were isolated from both control mice and C5aR siRNA virus-challenged mice, and their ability to produce TNF-α when treated with immune complexes in vitro was assessed; n = 6–8 mice/group.