Figure 5.

Flow cytometric analysis of in vivo C5aR-siRNA silencing on C5aR expression in alveolar macrophages. BAL fluids from mice infected with control virus or C5aR-siRNA virus were isolated as described in Materials and Methods. Alveolar macrophages, which represent ∼80–90% of cell population in BAL fluids, were identified by positive staining for F4/80. Expression of C5aR on alveolar macrophages was examined under both nonpermeabilized (A) and permeabilized conditions (B). Overlay analysis showed that expression of C5aR on alveolar macrophages was not changed by in vivo C5aR-siRNA silencing. Experimental data represent measurements from ≥4 mice/group.