Figure 6.

Flow cytometric analysis of in vivo C5aR-siRNA silencing on C5aR expression in interstitial macrophages. Whole-lung leukocytes were isolated as described in Materials and Methods. As shown by F4/80 gating, interstitial macrophages represent ∼10–15% of whole-lung leukocytes. Lung leukocytes were treated with or without 0.1% saponin to examine C5aR expression in interstitial macrophages under both nonpermeabilized (A) and permeabilized (B) conditions. No change of C5aR expression was found among groups under both conditions. Experimental data represent measurements from ≥4 mice/group.