Figure 5.

Preventing Drp1 SUMOylation does not alter its translocation to mitochondria. HeLa cells were transfected with Myc-tagged, WT Drp1 variant 3 or the 4 KR mutant form that cannot be SUMOylated. A) Total cell lysates were immunoblotted for Myc. HSP60 levels were monitored on the same immunoblot to ensure equal protein loading. B) Cells expressing WT or 4 KR mutant Drp1 were either left untreated or else treated with staurosporine (STS) in the presence of zVAD-fmk for 8 h. Mitochondria were isolated; equal protein aliquots were resolved by SDS-PAGE, then immunoblotted for Myc and HSP60.