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GBS strains, plasmids, and primers used in this study
| Strain or plasmid | Genotype/phenotype | Reference |
|---|---|---|
| COH1 | cpsIII, Tetr/wild type | 32 |
| COH1-13 | COH1, Tn916 E cpsE/CPS Tetr Emr | 33 |
| COH1-350 | COH1; cpsK Tetr Cmr | 34 |
| E. coli K1 BL21 | F− ompT hsdS (r− m−) gal dcm | |
| Plasmid vectors | ||
| pET-32a(+) | Promoter: T7 lac; tags: Trx His; selection: Amp | |
| Primers | ||
| Forward sht (shtfor) | GGAGGACCATGGTGAAGAAAACATATGGTTA | |
| Reverse sht (shtrev) | GGAGGACTCGAGAGGGTTTATTTGTTGA |
