Fig. 3.

Dot6 and Tod6 are redundantly required for Ribi gene repression after nutrient deprivation. The indicated prototrophic strains were grown in synthetic medium containing 2% glucose and 0.1% glutamine as sole carbon and nitrogen sources, respectively, to an OD₆₀₀ of 0.25, harvested by filtration nitrocellulose membranes via rapid vacuum filtration, washed with corresponding nutrient starvation medium, then transferred to identical prewarmed synthetic medium lacking glutamine (A–C; Nitrogen Starvation) or lacking glucose (D–F; Glucose Starvation). Samples were collected immediately before transfer (time point 0) and at 20 and 80 min after transfer and were analyzed by expression microarrays as described in Materials and Methods and the legend to Fig. 2. The resulting data are displayed as scatter plots with the log₂ expression level of each gene 80 min after transfer relative to that before transfer in a mutant strain versus the corresponding value for that gene in the WT strain (Y3628). (A and D) dot6 strain (Y3714). (B and E) tod6 strain (Y3715). (C and F) dot6 tod6 strain (Y3716).