Spatial expression pattern of G1 mRNA and function of G1 protein. (A and B) in situ localization of G1 mRNA. fm, floret meristem; le, lemma; pa, palea; sl, sterile lemma; st, stamen. (C) Subcellular localization of the G1-GFP fusion protein. (D) DAPI staining of the nucleus. (E) Merged image of C and D. (F) Subcellular localization of GFP. (G) Trans-activation analysis of G1 by a yeast GAL4 system. GAL4BD, DNA-binding domain of GAL4; 5× GAL4, 5 copies of GAL4-upstream activating sequence. (Scale bars: 50 μm.)