Figure 2.

Gene expression after TNF withdrawal (a) 3T3 fibroblasts were exposed to TNF (10 ng/ml) continuously for 10 hours (TNF; red line), or were exposed to TNF for 6 hours, after which TNF was washed away (TNF withdrawal; blue line). Expression of genes in each group was measured by qRT-PCR at indicated time points. The data are mean ± s.d. of triplicate samples and are representative of three to six independent experiments with similar results. (b). 3T3 fibroblasts were stimulated with medium alone (Medium) or with TNF for 6 hours (TNF), or were exposed to TNF for 6 hours after which TNF was washed away and cells were incubated in medium for an additional 18 hours (TNF withdrawal). CCL2 and CCL5 protein in supernatants were measured by ELISA. Note that the production of CCL2 and CCL5 in medium alone was too low (approximately 20 pg/ml) to be shown and CCL5 production is delayed in kinetics and thus lower comparing with CCL2. The data are mean ± s.d. of triplicate samples and representative of three independent experiments with similar results.