Table 1.
mRNA half life in mouse cells
| Genes | AREs in 3'UTR | mRNA half life ( hour, mean ± s.d., n=3 ) |
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|---|---|---|---|---|---|---|---|---|---|
| 3T3 | BMDM | ||||||||
|
|
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| Basal | TNF (0.5h) | TNF (6h) | Basal | TNF (0.5h) | TNF (6h) | LPS (0.5h) | LPS(6h) | ||
| Group I | |||||||||
| Fos | 10 | 0.22 ± 0.04 | 0.17 ± 0.02 | TLTD | 0.14 ± 0.01 | 0.08 ± 0.02 | 0.16 ± 0.05 | 0.29 ± 0.02 | 0.20 ± 0.04 |
| Tnf | 10 | TLTD | TLTD | TLTD | 0.17 ± 0.05 | 0.11 ± 0.01 | 0.13 ± 0.08 | 1.45 ± 0.17 | 0.47 ± 0.04 |
| Zfp36 | 8 | 0.28 ± 0.03 | 0.18 ± 0.05 | 0.28 ± 0.02 | 0.23 ± 0.02 | 0.10 ± 0.01 | 0.20 ± 0.06 | 3.70 ± 0.10 | 0.45 ± 0.04 |
| Edn1 | 6 | 0.34 ± 0.12 | 0.31 ± 0.03 | 0.50 ± 0.10 | 0.46 ± 0.07 | 0.40 ± 0.11 | 0.38 ± 0.16 | stable | 0.31 ± 0.05 |
| Tnfaip3 | 6 | 0.28 ± 0.05 | 0.32 ± 0.08 | 0.32 ± 0.05 | 0.23 ± 0.05 | 0.12 ± 0.01 | 0.19 ± 0.07 | 0.55 ± 0.19 | 0.27 ± 0.04 |
| Nfkbia | 5 | 0.53 ± 0.06 | 0.48 ± 0.09 | 0.49 ± 0.12 | 0.28 ± 0.04 | 0.19 ± 0.02 | 0.21 ± 0.02 | 0.52 ± 0.12 | 0.29 ± 0.06 |
| Il1b | 5 | TLTD | TLTD | TLTD | 0.26 ± 0.10 | 0.27 ± 0.13 | 0.75 ± 1.17 | stable | stable |
| Cxcl1 | 7 | 0.64 ± 0.08 | 0.51 ± 0.07 | 0.94 ± 0.26 | 0.16 ± 0.06 | 0.15 ± 0.06 | 0.25 ± 0.10 | stable | 1.74 ± 1.11 |
| Cxcl2 | 7 | 0.54 ± 0.17 | 0.50 ± 0.11 | 0.68 ± 0.31 | 0.16 ± 0.04 | 0.17 ± 0.05 | 0.19 ± 0.01 | stable | 5.31 ± 4.36 |
| Irf1 | 4 | 0.74 ± 0.20 | 0.73 ± 0.09 | 0.68 ± 0.09 | 0.47 ± 0.12 | 0.40 ± 0.03 | 0.38 ± 0.09 | 4.16 ± 4.09 | 0.49 ± 0.11 |
| Group II | |||||||||
| Ccl20 | 4 | 1.46 ± 0.37 | 2.28 ± 0.93 | 2.78 ± 1.20 | TLTD | TLTD | TLTD | TLTD | TLTD |
| Ccl2 | 2 | 1.04 ± 0.08 | 1.37 ± 0.30 | 1.29 ± 0.26 | 0.39 ± 0.08 | 0.36 ± 0.04 | 0.61 ± 0.38 | stable | 1.09 ± 0.69 |
| Cxcl10 | 2 | 4.84 ± 2.59 | 5.06 ± 4.63 | 3.85 ± 0.31 | 0.77 ± 0.38 | 1.16 ± 0.70 | 1.03 ± 0.78 | 2.03 ± 1.83 | 2.06 ± 0.38 |
| Icam1 | 2 | 4.19 ± 1.90 | > 8.0 | 5.37 ± 1.83 | 1.52 ± 0.89 | 6.56 ± 1.33 | 2.94 ± 1.53 | stable | 6.25 ± 2.41 |
| Nfkbie | 1 | 4.48 ± 0.74 | > 8.0 | 2.88 ± 1.17 | |||||
| Vcam1 | 2 | 7.69 ± 1.04 | > 8.0 | > 8.0 | |||||
| Mmp10 | 1 | > 8.0 | > 8.0 | > 8.0 | |||||
| Group III | |||||||||
| Mmp13 | 2 | stable | stable | stable | 2.92 ± 2.56 | 5.71 ± 3.30 | stable | stable | stable |
| C3 | 0 | stable | stable | stable | |||||
| Mmp3 | 1 | stable | stable | stable | TLTD | TLTD | TLTD | TLTD | TLTD |
| Ccl5 | 0 | stable | stable | stable | stable | stable | stable | stable | stable |
| Saa3 | 0 | stable | stable | stable | stable | stable | stable | stable | stable |
| Serpina3g | 0 | stable | stable | stable | |||||
| House-keeping | |||||||||
| Actb | stable | stable | stable | ||||||
| Rpl32 | stable | stable | stable | ||||||
| Rpl13 | stable | stable | stable | ||||||
mRNA half lives are presented, determined as described in Methods. TLTD means too low to be determined