Table 5.

Nicotine and Metabolites in Meconium by LC-APCI-MS/MS: Stability Data

	% of freshly extracted quality control concentrations (N=5)
	24 h room temperature			72 h 4°C
	Low	Medium	High	Low	Medium	High
OH-cotininea	103.2	76.2	98.4	87.2	96.6	103.6
Cotinine	97.4	86.8	95.8	94.8	93.2	104.4
Norcotinine	106.8	79.5	94.0	102.2	95.0	102.4
Nornicotine	90.1	74.5	93.6	89.6	88.0	96.5
Nicotine	97.6	74.5	97.3	94.1	99.6	119.0
	3 freeze thaw cycles			24 h 15°C autosampler
	Low	Medium	High	Low	Medium	High
OH-cotinine	106.0	82.3	97.2	91.2	100.6	101.1
Cotinine	100.4	85.3	94.0	100.2	103.2	100.1
Norcotinine	97.9	83.4	94.1	96.2	100.3	102.5
Nornicotine	97.8	73.9	88.1	87.9	103.0	108.3
Nicotine	75.1	97.1	120.4	102.9	98.4	101.6

^atrans-3’-Hydroxycotinine