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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Methods. 2009 Jan 11;6(2):135–138. doi: 10.1038/nmeth.1293

Figure 1.

Figure 1

17-ODYA labeling and detection of palmitoylated proteins. (a) Schematic of 17-ODYA labeling. Cultured Jurkat T-cells were metabolically labeled with 17-ODYA and the lysates were then reacted with rhodamine-azide or biotin-azide for gel-based and LC-MS-based characterization of palmitoylated proteins. (b) Profiling palmitoylated proteins in the membrane fraction of Jurkat T-cells incubated with 25 µM 17-ODYA for 8 hours. Half of the sample was boiled in 2.5% hydroxylamine (NH2OH) for 5 minutes to hydrolyze thioesters and remove 17-ODYA labeling.