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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr;233(4):447–455. doi: 10.3181/0708-RM-230

Figure 3.

Figure 3

CS accelerates the differentiation of osteoblasts. (A) Osteoblast differentiation in CS-supplemented cultures showed enhanced ALP-positive osteoblasts at day 10 of culture, more oil-forming adipocytes (arrow) at day 14 of culture, and and stronger Alizarin red S–stained bone tissue at day 28 of culture. The CS influence on osteoblast differentiation was as effective as that of dexamethasone (1 μg/ml). (B) The time course of the appearance of ALP-positive osteoblasts in these osteoblast differentiation cultures with or without CS (500 μg/ml) was assessed. Cells were stained for ALP after 7, 10, and 14 days, and the numbers of ALP-positive cells per 100 cells were counted. CS enhanced the number of ALP-positive cells at all time points. (C) Oil-forming adipocytes were counted (30 fields) at 7, 10, 14, 21, and 28 days. CS promoted differentiation of adipocytes earlier than did control. Data and error bars represent the means ± 1 SEM for one representative of three experiments, as analyzed by GraphPad Prism software version 3.03. *, P < 0.01 by Student’s t test (compared with control). A color figure is available in the online version of the journal.

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