Figure 5.
Influence of CS on osteoclast differentiation, (A) A CS dose-dependent reduction of tartrate-resistant acid phosphatase (TRAP)-stained cells was found in 7-day BM cultures. (B) The number of osteoclasts, evidenced by multinucleated giant cells containing more than three nuclei (insert), were counted and plotted against CS concentration. A reverse dose-dependent fashion was found. Values are means ± 1 SEM of six determinations. One represented data from three repeated experiments were shown. *, P < 0.01 by ANOVA (compared with control without CS addition).