Fig. 2.

Low magnification electron micrograph of D-aspartate uptake in a rat hippocampal slice. Hippocampal slices were incubated in D-aspartate (50 μM, 3 min), fixed and labeled with antibodies to glutaraldehyde-fixed D-aspartate. Nerve terminals (t) and glia (g) were heavily labeled (note high number of black uniformly sized dots, three of which are indicated by arrows). Dendrites (d) and dendritic spines (s) were virtually unlabeled. Arrowheads indicate the location of a synaptic cleft. Note the large white areas which are not bounded by any continuous membranes and therefore probably represent extracellular space (es). Scale bar=300 nm.