Table 2.

Synaptosomes: density of D-aspartate labeling in terminals compared to glia

	Incubation time with 50 μM D-Asp
	0 s	10 s	60 s	20 min
Gold particles over terminals	0.3	1.8	10.5	42.4
Gold particles over non-terminals	0.7	0.8	4.9	9.1
Labeled terminals as percent of total number of terminals	Not counted	Not counted	76%	68%
Relative density of gold particles over terminals per unit area	1	6.7	32	160

Labeling of synaptosomes with anti-D-aspartate antibodies after differing durations of exposure to 50 μM D-aspartate. Data in the first two rows were obtained by analyzing 8 to 10 random pictures of synaptosome sections from each treatment and are presented as mean number per picture over each area. The relative area of synaptosomal profiles was obtained using a point-density mapping method and used to normalize the density relative to the density at 0 s, which represents no exposure and thus background levels.