Table 2.
Influence of thrombopoietin or interleukin-5 on the number or mean cell content of blast colonies stimulated by stem cell factor
| Experiment number | Stimulus
|
||
|---|---|---|---|
| SCF | SCF + TPO | SCF + IL-5 | |
| 1 | 13* | 18 | 15 |
| 2 | 25* | 25 | |
| 3 | 24* | 20 | |
| 4 | 14* | 17 | |
| 5 | 13* | 14 | |
| 6 | 12* | 11 | |
| 7 | 21* | 22 | |
| 1 | |||
| Dispersed | 1,310† | 810 | 1,170 |
| Multicentric | 6,220† | 6,680 | 6,960 |
| 2 | |||
| Dispersed | 250† | 440 | – |
| Multicentric | 1,760† | 2,470 | – |
Cultures of 50,000 C57BL bone marrow cells were stimulated for 7 days by 100 ng of SCF with or without 50 ng of TPO or 10 ng of IL-5. Data shown are mean blast colony counts from duplicate cultures or mean blast colony cell counts from pools of 10–30 colonies of each type.
*Blast colony number.
Mean cells per blast colony.