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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Nature. 2007 Jun 21;447(7147):1007–1011. doi: 10.1038/nature05883

Figure 3. dmPGE2 modulates colony number and haematopoietic differentiation in mouse embryonic stem cells.

Figure 3

M3434 and OP9 embryonic stem cell colony-forming assays were performed; counts are per 100,000 cells plated. An asterisk (*) indicates a statistically significant difference (two-tailed t-test; mean, s.d. and n listed in Supplementary Table 4). a, Effect of increasing doses of dmPGE2 and inhibition of cyclooxygenase activity by indomethacin on haematopoietic differentiation in methylcellulose; numbers of definitive erythroid (E), mixed granulocyte/monocyte (GM), and multi-potent (GEMM) progenitor colonies are shown (10 μM dmPGE2: GM, P = 0.005; GEMM, P = 0.017; 20 μM dmPGE2: E, P = 0.04; GM, P = 0.007; GEMM, P = 0.016; 100 μM indomethacin: GM, P = 0.024). b, Effect of dmPGE2 and indomethacin on OP9 haematopoietic colony number (20 μM dmPGE2, P = 0.047).