Skip to main content
. 2009 Nov 24;3(11):e553. doi: 10.1371/journal.pntd.0000553

Figure 1. Schematic representation of the M. ulcerans Agy99 megaplasmid pMUM001, and the promoterless GFP vector pSM20.

Figure 1

The regions upstream of key genes involved in mycolactone biosynthesis cloned into pSM20 and the names of the resulting plasmid GFP-reporter constructs are indicated. The 8.4 kb duplicated region that spans the load modules of mlsA1 and mlsB and 1.6 kb upstream of each gene is circled. E = EcoRV, B = BamHI, S = SphI, ‘*’ indicates that this single construct represents the encircled duplicated regions upstream of mlsA1 and mlsB.