Table 1. Comparison of mycolactone promoter sequences with mycobacterial consensus sequences.
| * Sequence | ||||||||
| Promoter | Species | Gene | −35 | Spacer | −10 | Spacer | +1 | Reference/comments |
| SigA | Mycobacteria | Consensus | TTGACW | 17 | TATAMT | 6 | G or A | [38],[40] |
| M. ulcerans | mlsA1/mlsB | TTGCAA | 19 | TAAATT | 13 | T | Predicted by PoSSuM: P-value 1.875074e-04 | |
| M. ulcerans | mlsA1/mlsB | TTGCAA | CCCATT† | Mutagenesis of -10 box reduces transcription | ||||
| M. ulcerans | mup045 | TCGACT | 16 | TACAGC | 3 | G | Predicted by PoSSuM: P-value 4.993513e-04 | |
| M. ulcerans | mup045 | TCGACT | GGGCCC† | Mutagenesis of -10 box reduces transcription | ||||
| SigD | Mycobacteria | Consensus | GTANCGSS | 16–20 | NNANNN | 1 | G | [40] |
| M. ulcerans | mup053 | GTATCGAC | 17 | ACAGGC | 6 | G | Predicted by PoSSuM: P-value 8.445395e-05 | |
| M. ulcerans | mup053 | GTATCGAC | GCCCGC† | Mutagenesis of -10 box has no effect |
*: R denotes A or G, S denotes C or G, M denotes A or C, W denotes A or T as per IUB ambiguity codes, N denotes any A, T, G or C.
†: Changed nucleotides shown in underline.