Figure 7.

Variation in the expression level of death receptors and their ligands induced by CSFV infection. The expression of death receptors and their ligands was evaluated either at the RNA level (A–E) or at the protein level (F). (A–E) Expression modulations induced in PBMC by Eystrup and Paderborn strain infection were assessed by real-time RT-PCR. (A), (B) and (C) for the death receptor ligands: TRAIL, FASL and TNFa and (D) and (E) for their respective receptors: DR4 and FAS. The presented PCR values are the Log₂ Ratios calculated from the CT results on D1, D2 and D3 pi and related to D0 as described in Materials and methods. (F) TNFa levels in serum were quantified by ELISA. Values are presented in relation to the levels quantified before infection. All data correspond to the mean (± SE) of results obtained for the 8 pigs infected by Eystrup or the 8 pigs infected by Paderborn. * p < 0.05 and ** p < 0.01 either compared each post-infection value with the respective D0 value using Paired Two-sample Student’s t-test, or Eystrup values with Paderborn values using Two-sample Student’s t-test at each infection time-point.