Figure 5.
Nucleotide-binding sites. (A) The bottom view of V1-ATPase. The difference (Fnucleotide−Ffree) map is shown as blue surfaces contoured at the +5.0σ level. The catalytic sites of the A subunits are indicated by red circles and possible nucleotide-binding sites of B subunits are indicated by blue circles. (B) The subunit interface between AN and BN. The difference map is shown as blue and yellow meshes contoured at the +5.0σ and −4.0σ levels, respectively. The side chain of Arg 360 and the model of ADP are drawn by the superposition of the structure of the F1-ATPase (Menz et al, 2001). (C) The subunit interface between AN′ and BN′. (D) The subunit interface between AW and BW.