Figure 2.
Dynamics of CD45 distribution during the formation of the immunological synapse. T cells were labeled with I3/2.3-Cy5 and H57-Alexa546 and settled onto bilayers incorporating unlabeled ICAM-1, and Oregon green-conjugated MHC-peptide. (Scale bars = 10 μm.) (A) Synapse formation at the T cell/bilayer interface. TCR (red), MHC-peptide (green), CD45 (blue). (B) Red/green overlays of TCR and CD45 distribution during the formation of the immunological synapse on three representative cells. TCR (red), CD45 (green).