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SDS-PAGE following the purification of (A) Trx-ELP and (B) ELP-Trx stained with Coomassie blue. Lane 1 on both gels: molecular weight markers (MW); Lane 2: lane soluble cell lysate (L); Lane 3: the resuspended pellet from the first hot spin (P 1HS); Lane 4: the supernatant from the first cold spin (SN 1CS); Lane 5: the supernatant from the final, fifth cold spin (SN, FCS). The molecular weights of Trx-ELP and ELP-Trx are 50.0 kDa for both proteins.
