Figure 1.

(a) Representative surface plasmon resonance data. Epitope competition was established by saturating coupled HPV31 L1 VLP with H31.F16 MAb (crude ascite fluid), and the MAb saturation was verified by injection of the same MAbs (hybridoma supernatant). MAb binding competition was then established by successively injecting H31.B18, H31.D7, H31.E16, H31.E17, and H31.F7 MAbs (hybridoma supernatants). The biosensor was then regenerated by three injections of 30 mmol/L HCl. Epitope map constructed from the interaction studies between the 15 HPV31 MAbs that recognized conformation epitopes by SPR. (b) Antibodies that neutralized internalization are in black and those that neutralized cell attachment are in red. The H31.D24 antibody was non-neutralizing (in blue). (c) The antibodies that inhibited VLP binding to heparin are in red. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]