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Kinetic Parameters for Caspase-7 Alanine-Substitution Mutants
| Km (μM) | kcat (s−1) | 106 × [kcat/Km] (M−1s−1) | Relative activity (relationship factor of mutant kcat/Km versus WT) | |
|---|---|---|---|---|
| WT | 23 ± 2 | 0.35 ± 3×10−3 | 0.015 | 1.0 |
| Y211A | 18 ± 10 | 0.39 ± 3×10−3 | 0.021 | 1.4 |
| K212A | 210 ± 20 | 0.93 ± 4×10−2 | 0.004 | 0.26 |
| I213A | 480 ± 50 | 0.07 ± 5×10−3 | 1.5 × 10−4 | 0.01 |
| P214A | 8 ± 1 | 0.10 ± 4×10−3 | 0.012 | 0.8 |
| Y215A | 26 ± 8 | 0.70 ± 2×10−2 | 0.026 | 1.7 |
