Skip to main content
. 2009 Apr 29;18(7):1552–1563. doi: 10.1002/pro.150

Figure 4.

Figure 4

SDS-Page analysis of expression in E. coli of the human 11β-HSD1 mutants (R137C and K187N) in both wild-type (A) and F278E (B) backgrounds. (A) Insoluble (pellets) and soluble (supernatant) fractions from lysates of cultures expressing R137C and K187N mutants in the wild-type background. Expression levels of both recombinant proteins were low, and neither mutant was expressed in a soluble form. (B) Insoluble (pellets) and soluble (supernatant) fractions from lysates of cultures expressing the double mutants R137C/F278E and K187N/F278E. Arrows indicate position of the recombinant proteins. Although the protein produced was still largely insoluble, some soluble protein was evident in the supernatant for the F278E/R137C mutant.