Fig. 3.

Hyperthermia, alone or in combination with other danger signals, significantly increases SMX-protein adduct formation in human dendritic cells. Human monocyte-derived dendritic cells were incubated with SMX (2 mM) at 37 or 38°C, which significantly increased SMX adduct formation (n = 6; A). Finally, monocyte-derived dendritic cells were incubated with SMX (2 mM) in different combinations of danger signals, showing a significant enhancement in SMX adduct levels when hyperthermia was combined with LPS or PGE2 (n = 4; B).