Fig. 4.

Dimedone significantly decreases the formation of SMX-protein adducts in human dendritic cells. Human dendritic cells [monocyte-derived cells (n = 4) or cell lines HL60 (n = 7) and THP1 (n = 6)] were incubated with SMX (2 mM) in the presence or absence of dimedone (5 mM) that binds covalently to sulfenic acid cysteine, in both a physiological or danger conditions (PMA, 5 ng/ml or LPS, 100 ng/ml). Dimedone did not affect the average OD in DMSO controls but significantly reduced SMX-protein adduct formation in monocyte-derived dendritic cells and THP1, both in physiological and danger conditions. Conversely, dimedone only significantly decreased SMX adduct levels in LPS-stimulated HL60 cells. Results are presented as the average of blanked OD values from four independent experiments.