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Capsaicin pretreatment inhibits BoNT/A uptake into Neuro 2a cells. Representative fluorescence labeling of Neuro 2a cells with Alexa Fluor 647-BoNT/A for BoNT/A alone (A) and BoNT/A after CAP pretreatment (B). Left, BoNT/A was loaded by incubating cells with Alexa Fluor 647-BoNT/A as described in Materials and Methods. Right, corresponding brightfield images.
