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Capsaicin pretreatment of Neuro 2a cells prevents BoNT/A inhibition of 40 mM KCl-stimulated FM 1-43 uptake. A, confocal images of FM 1-43 labeling for control (left), BoNT/A alone (center), and 10 μM capsaicin pretreated (right) Neuro 2a cells. B. Average fluorescence intensity (arbitrary units; a.u.) ± S.E.M for control, BoNT/A alone, and capsaicin pretreated Neuro 2a cells. The number in each bar indicates the number of cells summarized in the mean value.
