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Mouse motor nerve endings and Neuro 2a cells express TRPV1. A is a representative Western blot for TRPV1 probed with anti-rabbit TRPV1 antibody in mouse hemidiaphragm, and Neuro 2a cell lysates as described in Materials and Methods. Lysate of rTRPV1 expressing HEK293 cells was used as control (HEKTRPV1). B and C, respectively, illustrate immunocytochemistry of TRPV1 expression in motor nerve endings (yellow arrows) of YFP mice and Neuro 2a cells (white arrows).
