Figure 1.

General approaches of quantitative proteomics. (a) Shotgun isotope labeling method. After labeling by light and heavy stable isotope, the control and sample are combined and analyzed by LC-MS/MS. The quantification is calculated based on the intensity ratio of isotope-labeled peptide pairs. (b) Label-free quantitative proteomics. Control and sample are subject to individual LC-MS/MS analysis. Quantification is based on the comparison of peak intensity of the same peptide or the spectral count of the same protein.