Fig. 5.

obgE mRNA expression levels increase after UV irradiation and rescues Δnos. (A) Quantitative real-time PCR was used to determine the ratios of obgE/gap in wild-type and Δnos cells before and following irradiation, and the induction levels of the overexpression strain Δnos:pObgE. The average ± SEM is shown for three independent experiments. (B) The complementation strain Δnos:pObgE was generated by introducing an IPTG inducible recombinant obgE gene on expression plasmid p11530. Δnos:pObgE cells were grown to OD ≈1.0 and either no IPTG, IPTG only before irradiation or IPTG prior and post-irradiation (throughout growth) was added to induce the ObgE gene. Cells were evaluated at 22 h after UV exposure.