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. 2009 Oct 13;106(43):18309–18314. doi: 10.1073/pnas.0708837106

Fig. 1.

Fig. 1.

Recombinant Ouro1 and Ouro2 proteins induce adult T-cell proliferation. (A) Schematic presentation of Ouro1 and Ouro2. Both proteins are predicted to contain central rod domains flanked by glycine-serine rich domains (GS). Bold lines with amino acid (AA) nos. represent sequences used for His-tagged recombinants. Y marks with AA nos. (red) show sequences used for raising specific antisera (see Results). (B) T-cell proliferation assay. Columns indicate the percentage of proliferating cells (mean ± SD from two to three independent experiments) cultured without a stimulus (WO) or with syngeneic larval tail tissues (Tail), Ouro1 recombinant protein (rOuro1), Ouro2 recombinant protein (rOuro2), or GFP recombinant protein (rGFP). The ANOVA test was used to assess statistical significance among values. *, P < 0.01. Significant differences are indicated by the Tukey's HSD test. n, number of assays; exp., number of experiments.