Fig. 4.

Knockdown of ouro1 and ouro2 gene expression results in retention of tail skin. (A) Antisense constructs for ouro1 and ouro2. Reverse-oriented ouro1 or ouro2 cDNA were placed after DsRed or gfp, respectively, which were driven by the hsp70 promoter. (B–E and E′) Suppression of tail regression in DsRed-anti-ouro1/gfp-anti-ouro2 DT tadpoles. Tails of DsRed-anti-ouro1/gfp-anti-ouro2 DT tadpoles (F₀) on days 1–14 after HS at stage 58/59 are shown by bright field and fluorescence microscopy (only GFP is shown). Note: DsRed-anti-ouro1/gfp-anti-ouro2 DT animals exhibit a pronounced delay in tail regression with a folded epidermis. (F–J and J′) gfp transgenic control. HS-induced gfp transgenic tadpole (line 9, F₂) show normal tail degeneration. Boxed areas in E′ and J′ are magnified for E and J, respectively.