Table 1.
Effects of overexpression of ouro genes on tail regression in transgenic tadpoles
| Transgene | Stage of HS | Line (F) | No. of HS tadpoles (nHS) | No. of GFP-expressing tadpoles (nG) | nG/nHS, % | Degenerated tails (nD) | nD/nG, % | P |
|---|---|---|---|---|---|---|---|---|
| Line 1 (F1) | ||||||||
| ouro1-gfp/ouro2-gfp | 57–59 | Line 2 (F2) | 55 | 43 | 78 | 31 | 72 | — |
| Line 3 (F2) | ||||||||
| Line 1 (F1) | ||||||||
| 50–52 | Line 2 (F2) | 38 | 27 | 71 | 0 | 0 | <0.01 | |
| Line 3 (F2) | ||||||||
| Line 4 (F1) | ||||||||
| ouro1-gfp | 58/59 | Line 5 (F1) | 74 | 37 | 50 | 0 | 0 | <0.01 |
| Line 6 (F2) | ||||||||
| ouro2-gfp | 58/59 | Line 7 (F2) | 36 | 20 | 56 | 0 | 0 | <0.01 |
| gfp | 57–59 | Line 8 (F1) | 97 | 72 | 74 | 0 | 0 | <0.01 |
| Line 9 (F2) |
F1 and F2 lines 1–9 were generated by mating their transgenic founders as described in Table S1 and Fig S6. GFP-expressing animals (nG) were selected from HS-treated specimens (nHS) on day 1 after HS as described in Materials and Methods. The phenotype ″Degenerated tails″ (nD) indicates that >50% of the GFP-expressing area disappeared by day 4 (around stages 61) as shown in Fig. 3E. The number of samples and phenotypes in the same experiment groups were combined (see Table S1 for details of each line). Note: HS treatment at the earlier stages (stages 50–52) for ouro1-gfp/ouro2-gfp double transgenic lines 1–3 did not initiate premature tail degeneration (see Results). P, one-way ANOVA, followed by Duncan's multiple range test against ouro1-gfp/ouro2-gfp DT tadpoles treated with HS at stage 57–59.