Table 2.
Effects of knockdown of ouro genes on tail metamorphosis in transgenic tadpoles
| Transgene | Stage of HS | F0 or line (F) | No. of HS tadpoles (nHS) | No. of GFP/DsRed-expressing tadpoles (nG) | nG/nHS, % | Retained tails (nR) | nR/nG, % | P |
|---|---|---|---|---|---|---|---|---|
| DsRed-anti-ouro1/gfp-anti-ouro2 | 58/59 | F0 | 144 | 12 | 8 | 7 | 58 | <0.01 |
| gfp-anti-ouro2 | 58/59 | F0 | 115 | 10 | 9 | 2 | 20 | <0.05 |
| gfp | F0 | 19 | 6 | 32 | ||||
| 57–59 | Line 8 (F1) | 73 | 56 | 77 | 0 | 0 | — | |
| Line 9 (F2) |
The number of samples and phenotypes in the same experiment groups were combined (see Table S2 for details of each F0 experiment or line). F1 and F2 gfp lines were generated as described in Fig. S6D. GFP-expressing animals (nG) were selected on day 1 from HS-treated tadpoles (nHS). Retained tails (nR) were counted on days 7–14 (stages 64–66). The same specimens of gfp lines 8 and 9 as in Table 1 were analyzed for tail retention. Note: the number of HS-tadpoles was reduced because some of tadpoles were killed for histochemical examination or died before being counted. P, one-way ANOVA, followed by Duncan's multiple range test against gfp.