Fig. 2.

The typical mechanochemical cycle resulting from our model is consistent with the kinetic model proposed in Rief et al. (20). (A and B) As each head undergoes kinetic state transitions (A), the myosin V dimer typically cycles through three distinct mechanical states, I–III (B). Color-coded boxes indicate the correspondence between mechanical states and kinetic states. (C) Data representative of hand-over-hand stepping as the motor undergoes the mechanochemical transitions illustrated in A and B, showing the time-dependent motion of each head domain (red and blue) and the neck domain juncture (black).