TABLE 1.
Capacitance values of PD and LP neurons obtained with different protocols
| Protocol | PD Neuron Capacitance, nF |
LP Neuron Capacitance, nF |
||||
|---|---|---|---|---|---|---|
| Average | SD | n | Average | SD | n | |
| CC_step | 65.61 | 68.23 | 29 | 83.82 | 50.71 | 18 |
| VC_step | 2.61 | 0.23 | 30 | 5.23 | 4.23 | 18 |
| VC_ramp (dV/dt = 0.02 mV/ms) | 3.61 | 1.23 | 29 | 5.48 | 3.41 | 18 |
| VC_ramp (dV/dt = 0.5 mV/ms) | 1.61 | 0.23 | 32 | 2.03 | 1.18 | 18 |
The average values of τm, τ1, R0, and Rin for the PD neuron were 158.7 ± 96.4 ms, 33.9 ± 17.0 ms, 2.3 ± 1.7 MΩ, and 11.4 ± 6.0 MΩ, respectively (n = 29). The average values of τm, τ1, R0, and Rin for the LP neuron were 215.2 ± 67.1 ms, 29.0 ± 10.3 ms, 2.1 ± 0.9 MΩ, and 9.7 ± 6.8 MΩ, respectively (n = 18). A post hoc analysis with Dunn's method showed that the CC_step estimate is significantly higher (P < 0.001) than that of either of the two voltage-clamp methods, and that the capacitance estimate with the fast voltage-clamp ramp method (0.5 mV/ms) is significantly (P < 0.001) different from that with either VC_step or slow (0.02 mV/ms) voltage-clamp ramp method. The capacitance estimates with VC_step and slow (0.02 mV/ms) voltage-clamp ramp protocols are not significantly different.