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. 1998 May 26;95(11):6413–6418. doi: 10.1073/pnas.95.11.6413

Figure 4.

Figure 4

RT–PCR analysis of total RNA from leukemic cells of patient 68 indicating MLL-hCDCrel chimeric mRNA. RT–PCRs with sense and antisense primers from MLL exon 6 and hCDCrel exon 3, respectively, and randomly primed cDNA template gave a single 247-bp product (Upper). Direct sequencing of the products of four reactions revealed an in-frame fusion of MLL exon 7 to hCDCrel exon 3 at position 142 of the 2,032-bp full-length hCDCrel cDNA (GenBank accession no. U74628) (16) (Lower). Reactions using β-actin primers and RNA negative control (distilled H2O) are also shown (Upper).