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. 2009 Nov 20;4(11):e7919. doi: 10.1371/journal.pone.0007919

Figure 4. Constitutive activation of Akt in macrophages modulates Fas expression in response to F. tularensis subsp. novicida infection.

Figure 4

(A) BMM isolated from wild type and Myr Akt mice were infected with F. tularensis subsp. novicida for the indicated time points. Protein-matched cell lysates were probed with Fas antibody and subsequently reprobed with actin antibody. The graph shows the mean and SD of Fas band intensities from three independent experiments (*** p<0.00058 for the comparison of wild-type and Myr Akt BMM). (B) Cell death of wild type and Myr Akt BMM was examined by measuring the amount of LDH release during infection (* p<0.0012 for 12 h and p<0.0015 for 24 h). The graph shows mean and SD of LDH release from three independent infections. (C) To confirm the expression of the Myr Akt transgene, cell lysates were analyzed by Western blotting with Akt antibody.