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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Curr Opin Biotechnol. 2009 Aug 31;20(4):486–491. doi: 10.1016/j.copbio.2009.08.005

Figure 2.

Figure 2

Directed evolution can be used to generate novel metabolic routes to useful chemicals. A phosphoenolpyruvate (PEP)-independent route (shown in black) to the key intermediate, 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP), was engineered by using directed evolution to enhance a weak DAHP-synthesizing activity present in 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase [23•]. The original route using DAHP synthase is shown in gray.