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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Chem Commun (Camb). 2007 Dec 19;(7):823–825. doi: 10.1039/b714446f

Fig. 1.

Fig. 1

(a) Absorption spectral changes of a 0.1 v/v% solution of BCG:BTB indicator in 50 mM mixed buffer (16.6 mM in potassium phosphate/BTP/Hepes) and 50% v/v glycerol from pH 6.0 to 8.6. Arrows indicate direction of increasing pH. (b) Apparent pH vs. temperature for selected buffers as probed by indicator dyes. Error bars indicate standard deviation of three replicates. Images show color change upon cooling from 25°C to −140°C in presence of each buffer. (BTP buffer not shown)