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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Chem Commun (Camb). 2007 Dec 19;(7):823–825. doi: 10.1039/b714446f

Fig. 2.

Fig. 2

(a) Degradation of oxacillin in different buffers at low temperatures. (b) HPLC chromatograms showing base catalyzed conversion of 100 μM oxacillin (Tr 22.5 min) to its hydrolysis product (Tr 17.8 min) over time at −20 °C in HEPES and TIP7 buffers. Samples in dark blue were thawed every 48 hrs and refrozen after analysis. Control solutions in light blue remained frozen until end of trial. All HPLC separations were performed at 25 °C while monitoring A220. All samples measured pH 7.00 ± 0.05 at 25 °C before and after storage.